|
|
|
|
- CHO, BHK, rat and human cell lines, growing in serum-and protein-free media
- Fully documented and compliant to regulatory requirements
- Good growth and expression behavior in different process systems
- Tested for absence of mycoplasma and viruses
| CHOSI |
|
BHK-SFS |
|
BHK-SF |
 |
|
 |
|
 |
| Single Cell Suspension |
|
Single Cell Suspension |
|
Adherent Monolayer |
Defined and Reproducible Culture Conditions with
- CHOSI, CHO Serum Independent
derived from CHO dhfr- (ATCC CRL-9096, deficient in dihydrofolate reductase) was
adapted for growth in serum- and protein-free, animal component free medium.
CHOSI grow as single cell suspension with a specific growth rate of about µ = 0.7
d-1, which corresponds to a doubling time of 1 per day
CHOSI can be cultured in several culture systems and are applicable as production
cell line for high level expression of recombinant proteins
CHOSI show high secretory activity and a human like glycosylation pattern
CHOSI can be transfected with high efficiency (up to 90 %)
- CHOSI-Ad, CHO Serum Independent, adherent
Genetic engineering of our well described CHOSI leaded to a permanent attachment of
the cells to glass and different plastic surfaces. This is useful for several cultivation
systems were adherent cells are needed (e.g. immobilization on microcarriers, chip
technology, assay development)
- CHOSI-dAd, CHO Serum Independent, inducible adherent
By introducing an inducible promoter system, we designed a cell line which can attached
and detached reversal. This makes it possible to generate a large number of indicator
cells for HTS or cell based systems by proliferating the cells in a bioreactor and
attach them to 96-well plates
- K-SI
- SF-BHK
- SF-BHK-Ad
- HEK-SI
- SF-HEPG2
We also offer adaptation of other cell lines to serum- and protein-free growth.
|
|
|
