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PROGENEX system for procaryotic gene expression |
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Based on our experience in eucaryotic gene expression systems for microbial
production of native as well as tagged proteins were developed.
Expression Vectors
- pLEGX (heat inducible lambda promoter)
- pEGXpress (synthetic promoter, RBS and Terminator)
- selection by Kan resistance or via complementation of strain auxotrophy
- show high plasmid stability even without selection pressure
- expression in inclusion bodies or as soluble protein
Host Strains
- E. coli B834 (protease deficient) or E. coli BL21 (high densities & stable)
- E. coli Exogen for soluble expression
- mutants with auxotrophic phenotypes
- secretory pathway adaptation to the protein of interest
Media
- fully synthetic media
- adapted to expression strain
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